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Rajbhar, Yogesh Prasad
- Effect of Different Dose of Cytokinin for Shoot Multiplication of Banana (Musa paradisiaca L.) Variety ‘GRAND NAINE’ under In-vitro Condition
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Authors
Yogesh Prasad Rajbhar
1,
Manmohan Singh
1,
Anil Kumar
1,
Gopal Singh
1,
Abhimanyu Singh
1,
D. K. Singh
1
Affiliations
1 Sardar Vallabhbhai Patel University of Agriculture Technology, Meerut (U.P.), IN
1 Sardar Vallabhbhai Patel University of Agriculture Technology, Meerut (U.P.), IN
Source
International Journal of Agricultural Sciences, Vol 12, No 1 (2016), Pagination: 65-72Abstract
The maximum callus formation (20.3%) was observed in treatment BAP 8 mgl-1 while the minimum (4.6%) was noted under control. With the combination of BAP and BA, the maximum callus formation ( 27.0%) was recorded under BAP 8 mgl-1 + BA 4 mgl-1 ; however, it was at par with BAP 8 mgl-1 + BA 3 mgl-1 at 75 days after inoculation.At 90 days after inoculation, maximum callus percentage (29.3) was found under BAP 8 mgl-1+ BA 4 mgl-1. At 105 days after inoculation, callus percentage was maximum callus (33.0%) was noted under the treatment combination of BAP 8 mgl-1+ BA 4 mgl-1, however, it was significantly at par with BAP 8 mgl-1+ BA 3 mgl-1, while the minimum (9.3%) was recorded under control again. The earliest shoot initiation (21.0, 22.0 days, respectively) was noted under BAP 8 mgl-1 and BA 5 mgl-1, separately; while it was statistically earliest i.e. 20.66 days in combination with BAP 2 mgl-1+ BA 5 mgl-1. Maximum shoot length (0.76 cm) was recorded in the treatment of BAP 8 mgl-1. at 20 days after shoot initiation.Maximum shoot length (3.06 cm) was noted under BAP 8 mgl-1 alone which statistically superior to other under BAP alone treatments while it recorded minimum under control at 40 days after shoot initiation. Under BA treatments, the maximum shoot length (2.26 cm) was noted with BA 4 mgl-1 and 5 mgl-1 both; however, it was at par with BA 2 mgl-1 and 3 mgl-1 at 40 days after shoot initiation. With the effect of BAP and BA combinations, the maximum shoot length (3.23cm) was recorded under BAP 8 mgl-1+ BA 5 mgl-1 at 40 days after shoot initiation. The minimum duration of ischolar_main initiation (14.66 days) was noted under the treatment of Indole Butyric acid 4 mgl-1 ; however, it was significantly at par with indole butyric acid 2 mgl-1 and 3 mgl-1. The maximum duration (34.33 days) was observed under control. Minimum number of ischolar_mains (4.0 ischolar_mains) were recorded under the treatment applied 1 mgl-1 IBA in culture medium. Further, number of ischolar_mains was found maximum 10.33 ischolar_mains under the treatment of 5 mgl-1 IBA followed by 4 mgl-1 IBA concentrations. Culture medium with IBA 5 mgl-1 showed maximum ischolar_main length (1.66 cm) followed by IBA 4 mgl-1 , 3 mgl-1 and 2 mgl-1 with 1.56 cm, 1.40 cm and 1.06 cm, respectively. It was concluded that BAP 8 mgl-1 and BA 5 mgl-1 separately performed better results on account of callus formation, shoot initiation and multiplication of shoots whereas with their combination viz., BAP 8 mgl-1 +BA 4 mgl-1 showed best result on the above parameters. For ischolar_main initiation and its development IBA 5 mgl-1 was found to be the best among all the treatments.Keywords
Cytokinin, Shoot Multiplication, Banana, In-vitro Condition.
References
- Darvari, F. M., Sariah, M., Puad, M. P. and Maziah, M. (2010). Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers. African J. Biotechnol., 9(16):2360-2366.
- Frison, E. and Sharrock, S. (1998). The economic, social and nutritional importance of banana in the world, pp 21 35. In: Bananas and Food Security (C. Picq, E. Foure and E. A Frison eds.). INIBAP, International Symposium, Douala, Cameroon.
- Harirah, A.A. and Khalid, N. (2006). Direct regeneration and RAPD assessment of male inflorescence derived plants of Musa acuminata cv. BERANGAN Asia-Pacific J. Molecul. Biol.& Biotechnol., 14 (1):11-17.
- Hernandez, J.B.P. and Garcia, P.R. (2008).Inflorescence proliferation for somatic embryogenesis induction and suspension-derived plant regeneration from banana (Musa AAA, cv. ‘DWARF CAVENDISH’) male flowers. Plant Cell Reports, 27 (6):965-971.
- Jafari, N., Othman, R.Y. and Khalid, N. (2011). Effect of benzylaminopurine (BAP) pulsing on in vitro shoot multiplication of Musa acuminata (banana) cv. BERANGAN. African J. Biotechnol.,10 (13):2446–2450.
- Krikorian, A.D., Irizarry, H., Cronauer-Mitra, S.S. and Rivera, E. (1993). Clonal fidelity and variation in plantain (Musa AAB) regenerated from vegetative stem and floral axis tips in vitro. Ann. Bot., 71 (6):519–535.
- Kumar, K.G., Krishna,V., Venkatesh and Pradeep, K. (2011). High Frequency regeneration of plantlets from immature male floral explants of Musa paradisica cv. PUTTABALE - AB Genome. Plant Tissue Cult. & Biotech., 21(2):199-205.
- Mahadev, S.R., Kathithachalam, A. and Marimuthu, M. (2011).An efficient protocol for large-scale plantlet production from male floral meristems ofMusa spp. cultivars VIRUPAKSHI and SIRUMALAI. In Vitro Cellular & Develop. Biol. Plant, 47 (5):611–617.
- Meenakshi, S., Shinde, B.N. and Suprasanna, P. (2011). Somatic embryogenesis from immature male flowers and molecular analysis of regenerated plants in banana "Lal Kela" (AAA). J. Fruit & Ornam. Plant Res.,19 (2):15–30.
- Rashid, K., Nezhadahmadi, A., Othman, R. Y., Ismail, N. A. , Azhar, S. and Efzueni (2012). Micropropogation of ornamental plant Musa beccarii through tissue culture technique using suckers and male buds as explants. Life Sci. J., 9 (4):2046–2053.
- Resmi, L. and Nair, A.S. (2007). Plantlet production from the male inflorescence tips of Musa acuminata cultivars from South India. Plant Cell Tiss. Organ Cult., 88:333-338.
- Sultan, M.T., Khan, M.H., Hakim, M .L., Mamun, A.N.K., Morshed, M.A. and Islam, M.R. (2011). In vitro plant regeneration from male flowers of banana.Internat. J. Biosciences, 1(1):1–11.
- Wirakarnain, S., Hossain, A.B.M.S. and Chandran, S. (2008). Plantlet production through development of competent multiple meristem cultures from male inflorescence of banana, Musa acuminta cv. ‘PISANG MAS’ (AA). American J. Biochem. & Biotechnol., 4 (4):325-328.
- Performance of High Density Planting of Mango (Mangifera indica L.) under Mid-Western Plain Zone of Uttar Pradesh
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Authors
Affiliations
1 Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), IN
1 Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), IN
Source
International Journal of Agricultural Sciences, Vol 12, No 2 (2016), Pagination: 298-301Abstract
An experiment was conducted at Zonal Research Centre -Nagina (Bijnor)-246 762(U.P.) during 2000 to evaluate the performance of high density planting of mango (Mangifera indica L.). The mango variety Dashehari scion wood of 20-22cm grafted on seedling ischolar_mainstock in July 1999 were planted at Horticulture Section in August, 2000 at two spacing viz., 10m × 10m (100 plants/ha-normal density) and 3.0m × 3.0 (1111plants/ha-high density). Five grafts were planted in normal density and 160 grafts were planted in high density employing about 0.1940 ha area. Grafts in normal density were planted at marked points prepared by pit digging (1×1×1m) and then by filling the pits with dug soil mixed with 100 g N, 75 g P2O5 and 75 g K2O fertilizer mixture, whereas grafts in high density were planted directly with the use of fertilizers containing 100 kg N, 75 kg P2O5 and 75kg K2O/ha. Normal package of pracices was applied in both the system of plantings. The plant height (5.30m) was recorded under normal planting whereas it was found little different in high density of mango at 11th year of their planting. The stem girth under normal system was noted as 55.7cm whereas,it was slightly reduced to 50.7cm under high density of planting. The expansion of East-West and North- South direction both were recorded same as 3.10m and 3.03m in normal planting and high density planting, respectively. The fruit yield 22.30q/ha was noted under normal density of planting whereas, it was 242.20q/ha at 5th year of planting under high density planting system. The fruit yield ranged from 22.30to 109.80 q/ha from 5th year to 11th year, respectively under normal system of planting whereas it ranged from 242.2 to 1093.22q/ha under HDP system of planting. The observations showed that the fruit yield ratio of normal system of planting to HDP were 1:10.86, 1:9.92, 1:9.90,1:8.67, 1:9.80, 1:9.27 and 1:9.95 from 5th year to 11th year of age, respectively. So, it is a very informative and need of the hour to plant HDP system of mango just to get 9 to 10 times more yield for increasing mango productivity and to reach the king of fruit to the common people.Keywords
High Density Planting, Mangifera indica, Mango.References
- Iyer, C.P.A. and Kurian, Reju M. (2006). High density planting in tropical fruits : Principles and practices. International Book Distributing, Lucknow ISBN 81-8189-0949.
- Majumder, P.K., Sharma, D.K. and Singh, R.N. (1982). A study on “high density” orcharding in mango (Mangifera indica L.) var. Amrapali. Punjab Hort. J., 22 : 123-127.
- Oosthuyse, S.A. (1992b). Ideas on pruning of mango trees. SA Mango Growers’ Assoc. Yearbook 12. pp. 1-7.
- Ram, S. and Sirohi, S.C. (1985). Studies on high density orcharding in mango cv. DASHEHARI. Acta Hort., 231 : 339-344.
- Ram, S., and Sirohi, S.C. (1991). Feasibility of high density orcharding in Dashehari mango. Acta Hort., 291 : 207-211.
- Ravishankar, H., Nalawadi, U.G., and Rao, M.M. (1992). Smoothing of alternate bearing rhythm in “Alphonso” mango by period and intensity of pruning under mildtropical rainy climatic conditions. IV Internat. Mango Symp. Abstrs., 86 p.